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Lorent, C., V. Pelmenschikov, S. Frielingsdorf, J. Schoknecht, G. Caserta, Y. Yoda, H. Wang,K. Tamasaku, O. Lenz, S. P. Cramer,M. Horch, L. Lauterbach & I. Zebger
Exploring structure and function of redox intermediates of [NiFe]-hydrogenases by an advanced experimental approach for solvated, lyophilized and crystallized metalloenzymes.
Angew. Chem. Int. Ed. Engl. in press (2021).
doi: 10.1002/ange.202100451

Harris, T., N. Heidary, S. Frielingsdorf, S. Rauwerdink, A. Tahraoui, O. Lenz, I. Zebger & A. Fischer
Electrografted interfaces on metal oxide electrodes for enzyme immobilisation and bioelectrocatalysis.
ChemElectroChem. in press (2021).
doi: 10.1002/celc.202100020

Caserta, G., V. Pelmenschikov, C. Lorent, A. F. T. Waffo, S. Katz, L. Lauterbach, J. Schoknecht, H. Wang, Y. Yoda, K. Tamasaku, M. Kaupp, P. Hildebrandt, O. Lenz, S. P. Cramer & I. Zebger
Hydroxy-bridged active site states of [NiFe]-hydrogenase unraveled by cryogenic vibrational spectroscopy and DFT computations.
Chem. Sci. 12:2189-2197 (2021).
doi: 10.1039/D0SC05022A



G. Caserta, C. Lorent, V. Pelmenschikov, J. Schoknecht, Y. Yoda, P. Hildebrandt, S. P. Cramer, I. Zebger & O. Lenz
In vitro assembly as a new tool to investigate catalytic intermediates of [NiFe]-hydrogenase.
ACS Catal. 10:13890-13894 (2020).
doi: 10.1021/acscatal.0c04079  

Claassens, N. J., G. Scarinci, A. Fischer, A. I. Flamholz, W. Newell, S. Frielingsdorf, O. Lenz & A. Bar-Even
Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle
Proc. Natl. Acad. Sci. U. S. A. 117:22452-22461  (2020).
doi: 10.1073/pnas.2012288117

Preissler, J., H. A. Reeve, T. Zhu, J. Nicholson, K. Urata, L. Lauterbach, L. L. Wong, K. A. Vincent & O. Lenz
Dihydrogen‐driven NADPH recycling in imine reduction and P450‐catalyzed oxidations mediated by an engineered O2‐tolerant hydrogenase.
ChemCatChem. 12:4853-4861  (2020).
doi: 10.1002/cctc.202000763

Al-Shameri, A., S. J. Willot, C. E. Paul, F. Hollmann, & L. Lauterbach
H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions.
Chem. Commun. (Camb.). 56:9667-9670 (2020).
doi: 10.1039/d0cc03229h

Fan, Q., P. Neubauer, O. Lenz & M. Gimpel
Heterologous hydrogenase overproduction systems for biotechnology – an overview
Int. J. Mol. Sci. 21:E5890 (2020).

Apfel, U.-P., W. Weigand, M. Horch, I. Zebger, O. Lenz and T. Fujishiro
Hydrogen development
In Bioorganometallic Chemistry (Weigand, M., Apfel, U.-P., eds.), pp. 13–136 (2020).
De Gruyter, Berlin
doi: 10.1515/9783110496574-002

Al-Shameri A, Petrich MC, Junge Puring K, Apfel UP, Nestl BM, Lauterbach L.
Powering artificial enzymatic cascades with electrical energy.
Angew. Chem. Int. Ed. Engl. 59:10929-10933 (2020).
doi: 10.1002/anie.202001302

Lenz, O.
Hydrogen comes alive.
Nat. Energy 5:426–427 (2020).
doi: 10.1038/s41560-020-0615-8

Caserta, G., C. Lorent, A. Ciaccafava, M. Keck, R. Breglia, C. Greco, C. Limberg, P. Hildebrandt, S. P. Cramer, I. Zebger & Oliver Lenz
The large subunit of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha – a minimal hydrogenase?
Chem. Sci. 11:5453-5465 (2020).
doi: 10.1039/D0SC01369B

Hartmann, S., S. Frielingsdorf, G. Caserta & O. Lenz
A membrane-bound [NiFe]-hydrogenase large subunit precursor whose C-terminal extension is not essential for cofactor incorporation but guarantees optimal maturation.
Microbiologyopen 9:1197-1206 (2020).
doi: 10.1002/mbo3.1029

Rowbotham, J. S., M. A. Ramirez, O. Lenz, H. A. Reeve & K. A. Vincent
Bringing biocatalytic deuteration into the toolbox of asymmetric isotopic labelling techniques.
Nat. Commun. 11, 1454 (2020)
doi: 10.1038/s41467-020-15310-z

Schulz, A. C., S. Frielingsdorf, P. Pommerening, L. Lauterbach, G. Bistoni, F. Neese, M. Oestreich & O. Lenz.
Formyltetrahydrofolate decarbonylase synthesizes the active site CO ligand of O2-tolerant [NiFe] hydrogenase.
J. Am. Chem. Soc. 142:1457-1464 (2020).
doi: 10.1021/jacs.9b11506
Recommended by the Faculty of 1000



Poladyan, A., S. Blbulyan, M. Sahakyan, O. Lenz & A. Trchounian
Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity.
Microb. Cell. Fact. 18:201 (2019).
doi: 10.1186/s12934-019-1251-5

Horch, M., J. Schoknecht, S. L. D. Wrathall, G. M. Greetham, O. Lenz & N. T. Hunt
Understanding the structure and dynamics of hydrogenases by ultrafast and two-dimensional infrared spectroscopy.
Chem. Sci. 10:8981-8989 (2019).
doi: 10.1039/c9sc02851j 

Borlinghaus, N., L. Weinmann, F. Krimpzer, P. N. Scheller, A. Al‐Shameri, L. Lauterbach, A.‐S. Coquel, C. Lattemann, B. Hauer, B. M. Nestl
Cascade biotransformation to access 3‐methylpiperidine in whole cells.
ChemCatChem. 11:1 – 6 (2019).
doi: 10.1002/cctc.201900702

Wilkening, S., F. J. Schmitt, O. Lenz, I. Zebger, M. Horch & T. Friedrich
Discriminating changes in intracellular NADH/NAD+ levels due to anoxicity and H2 supply in R. eutropha cells using the Frex fluorescence sensor.
Biochim. Biophys. Acta Bioenerg. 1860:148062 (2019).
doi: 10.1016/j.bbabio.2019.148062 

Lauterbach, L. & O. Lenz
How to make the reducing power of H2 available for in vivo biosyntheses and biotransformations.
Curr. Opin. Chem. Biol. 49:91-96 (2019).
doi: 10.1016/j.cbpa.2018.11.020

Al-Shameri, A., N. Borlinghaus, L. Weinmann,  P. N. Scheller,  B. M. Nestl & L. Lauterbach
Synthesis of N-heterocycles from diamines via H2-driven NADPH recycling in the presence of O2.
Green Chem. 21:1396-1400 (2019).
doi: 10.1039/C8GC03798A



Lenz, O., L. Lauterbach & S. Frielingsdorf
O2-tolerant [NiFe]-hydrogenases of Ralstonia eutropha H16: Physiology, molecular biology, purification, and biochemical analysis.
Methods Enzymol. 613:117-151 (2018).
doi: 10.1016/bs.mie.2018.10.008 

Hartmann, S., S. Frielingsdorf, A. Ciaccafava, C. Lorent, J. Fritsch, E. Siebert, J. Priebe, M. Haumann, I. Zebger & O. Lenz
O2-tolerant H2 activation by an isolated large subunit of a [NiFe] Hydrogenase.
Biochemistry 57:5339-5349 (2018).
doi: 10.1021/acs.biochem.8b00760 [PubMed]

Harris, T. G. A. A., N. Heidary, J. Kozuch, S. Frielingsdorf, O. Lenz, M. A. Mroginski, P. Hildebrandt, I. Zebger & A. Fischer.
In situ spectroelectrochemical studies into the formation and stability of robust diazonium-derived interfaces on gold electrodes for the immobilization of an oxygen-tolerant hydrogenase.
ACS Appl. Mater. Interfaces 10:23380-23391 (2018).
doi: 10.1021/acsami.8b02273 [PubMed]

Kalms, J., A. Schmidt, S. Frielingsdorf, T. Utesch, G. Gotthard, D. von Stetten, P. van der Linden, A. Royant, M. A. Mroginski, P. Carpentier, O. Lenz & P.Scheerer
Tracking the route of molecular oxygen in O2-tolerant membrane-bound [NiFe] hydrogenase.
Proc. Natl. Acad. Sci. U. S. A. 115:E2229-E2237 (2018).
doi: 10.1073/pnas.1712267115 [PubMed]

Preissler, J., S. Wahlefeld, C. Lorent, C. Teutloff, M. Horch, L. Lauterbach, S. P. Cramer, I. Zebger & O. Lenz
Enzymatic and spectroscopic properties of a thermostable [NiFe]‑hydrogenase performing H2-driven NAD+-reduction in the presence of O2.
Biochim. Biophys. Acta - Bioenergetics.
1859:8-18 (2018).
doi: 10.1016/j.bbabio.2017.09.006 [PubMed]



Heath, G. R., M. Li, H. Rong, V. Radu, S. Frielingsdorf, O. Lenz, J. N. Butt & L. J. C. Jeuken
Multilayered lipid membrane stacks for biocatalysis using membrane enzymes.
Adv. Funct. Mater
. 27:1606265 (2017).
doi: 10.1002/adfm.201606265

Wilkening, S., F. J. Schmitt, M. Horch, I. Zebger, O. Lenz & T. Friedrich
Characterization of Frex as an NADH sensor for in vivo applications in the presence of NAD+ and at various pH values.
Photosynth. Res. 133:305-315 (2017).
doi: 10.1007/s11120-017-0348-0 [PubMed]

Oberthuer, D., J. Knoška, M. O. Wiedorn, K. R. Beyerlein, D. A. Bushnell, E. G. Kovaleva, M. Heymann, L. Gumprecht, R. A. Kirian, A. Barty, V. Mariani, A. Tolstikova, L. Adriano, S. Awel, M. Barthelmess, K. Dörner, P. L. Xavier, O. Yefanov , D. R. James, G. Nelson, D. Wang, G. Calvey, Y. Chen, A. Schmidt, M. Szczepek, S. Frielingsdorf, O. Lenz, E. Snell, P. J. Robinson, B. Šarler, G. Belšak, M. Maček, F. Wilde, A. Aquila, S. Boutet, M. Liang, M. S. Hunter, P. Scheerer, J. D. Lipscomb, U. Weierstall, R. D. Kornberg, J. C. Spence, L. Pollack, H. N. Chapman & S. Bajt
Double-flow focused liquid injector for efficient serial femtosecond crystallography.
Sci. Rep. 7:44628 (2017).
doi: 10.1038/srep44628 [PubMed]

Reeve, H. A., P. A. Ash, H. Park, A. Huang, M. Posidias, C. Tomlinson, O. Lenz & K. A. Vincent
Enzymes as modular catalysts for redox half-reactions in H2-powered chemical synthesis: from biology to technology.
Biochem. J. 474:215-230 (2017).
doi: 10.1042/BCJ20160513 [PubMed]

Tejwani, V., F.-J. Schmitt, S. Wilkening, I. Zebger, M. Horch, O. Lenz & T. Friedrich
Investigation of the NADH/NAD+ ratio in Ralstonia eutropha using the fluorescence reporter protein Peredox.
Biochim. Biophys. Acta - Bioenergetics. 1858:86–94 (2017).
doi: 10.1016/j.bbabio.2016.11.001 [PubMed]



Bürstel, I. , E. Siebert , S. Frielingsdorf , I. Zebger , B. Friedrich & O. Lenz
Synthesis of CO from the central one-carbon pool: origin of the carbonyl ligand in O2-tolerant [NiFe]-hydrogenase
Proc. Natl. Acad. Sci. U. S. A. 113:14722-14726 (2016).
doi: 10.1073/pnas.1614656113 [PubMed]

Monsalve, K., I. Mazurenko, C. Gutierrez-Sanchez, M. Ilbert, P. Infossi, S. Frielingsdorf, M. T. Giudici-Orticoni, O. Lenz & E. Lojou
Impact of carbon nanotube surface chemistry on H2 oxidation by membrane-bound O2-tolerant hydrogenases.
ChemElectroChem. 3:2179–2188 (2016).
doi: 10.1002/celc.201600460

Gee, L. B., C. Y. Lin, F. E. Jenney Jr., M. W. Adams, Y. Yoda, R. Masuda, M. Saito, Y. Kobayashi, K. Tamasaku, M. Lerche, M. Seto, C. G. Riordan, A. Ploskonka, P. P. Power, S. P. Cramer & L. Lauterbach.
Synchrotron-based nickel Mössbauer spectroscopy.
Inorg. Chem
. 55:6866-6872 (2016).
doi: 10.1021/acs.inorgchem.5b03004 [PubMed]

Lauterbach, L., L. B. Gee, V. Pelmenschikov, F. E. Jenney, S. Kamali, Y. Yoda, M. W. Adams & S. P. Cramer.
Characterization of the [3Fe-4S]0/1+ cluster from the D14C variant of Pyrococcus furiosus ferredoxin via combined NRVS and DFT analyses.
Dalton Trans
. 45:7215-7219 (2016).
doi: 10.1039/c5dt04760a [PubMed]

Kalms, J., A. Schmidt, S. Frielingsdorf, P. van der Linden, D. von Stetten, O. Lenz, P. Carpentier & P. Scheerer
Krypton derivatization of an O2-tolerant membrane-bound [NiFe] hydrogenase reveals a hydrophobic tunnel network for gas transport.
Angew. Chem. Int. Ed. Engl
. 55:5586-5590 (2016).
doi: 10.1002/anie.201508976 [PubMed]

Radu, V., S. Frielingsdorf, O. Lenz & L. J. Jeuken
Reactivation from the Ni-B state in [NiFe] hydrogenase of Ralstonia eutropha is controlled by reduction of the superoxidised proximal cluster.
Chem. Commun. (Camb.)
52:2632-2635 (2016).
doi: 10.1039/c5cc10382g [PubMed]

Schäfer, C., M. Bommer, S. E. Hennig, J. H. Jeoung, H. Dobbek & O. Lenz
Structure of an actinobacterial-type [NiFe]-hydrogenase reveals insight into O2-tolerant H2 oxidation.
Structure 24:285-292 (2016).



Reeve, H. A., L. Lauterbach, O. Lenz & K. A. Vincent
Enzyme-modified particles for selective bio-catalytic hydrogenation via H2-driven NADH recycling.

Chem. Cat. Chem.7:3480-3487 (2015).
doi: 10.1002/cctc.201500766 [PubMed]

Heidary, N., T. Utesch, M. Zerball, M. Horch, D. Millo, J. Fritsch, O. Lenz, R. von Klitzing, P. Hildebrandt, A. Fischer, M. A. Mroginski & I. Zebger
Orientation-controlled electro-catalytic efficiency of an adsorbed oxygen-tolerant hydrogenase.
PLoS One. 10:e0143101 (2015).
doi: 10.1371/journal.pone.0143101 [PubMed]

Lonsdale, T. H., L. Lauterbach, S. Honda Malca, B. M. Nestl, B. Hauer & O. Lenz
H2-driven biotransformation of n-octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase.
Chem. Commun. (Camb)
. 51:16173-16175 (2015).
doi: 10.1039/C5CC06078H [PubMed

Löwenstein, J., L. Lauterbach, C. Teutloff, O. Lenz & R. Bittl
The active site of the NAD+-reducing hydrogenase from Ralstonia eutropha studied by EPR spectroscopy.
J. Phys. Chem. B 119:13834-13841 (2015).
doi: 10.1021/acs.jpcb.5b04144 [PubMed]

Siebert, E., Y. Rippers, S. Frielingsdorf, J. Fritsch, A. Schmidt, J. Kalms, S. Katz, O. Lenz, P. Scheerer, L. Paasche, V. Pelmenschikov, U. Kuhlmann, M. A. Mroginski, I. Zebger & P. Hildebrandt
Resonance Raman spectroscopic analysis of the [NiFe] active site and the proximal [4Fe-3S] cluster of an O2-tolerant membrane-bound hydrogenase in the crystalline state.
J. Phys. Chem. B 119:13785-13796 (2015).
doi: 10.1021/acs.jpcb.5b04119 [PubMed]

Ash, P. A., J. Liu, N. Coutard, N. Heidary, M. Horch, I. Gudim, T. Simler, I. Zebger, O. Lenz & K. A. Vincent
Electrochemical and infrared spectroscopic studies provide insight into reactions of the NiFe regulatory hydrogenase from Ralstonia eutropha with O2 and CO.
J. Phys. Chem. B 119:13807-13815 (2015).
doi: 10.1021/acs.jpcb.5b04164 [PubMed]

Roncaroli, F., E. Bill, B. Friedrich, O. Lenz, W. Lubitz & M. E. Pandelia
Cofactor composition and function of a H2-sensing regulatory hydrogenase as revealed by Mössbauer and EPR spectroscopy.
Chem. Sci.
6:4495-4507 (2015).
doi: 10.1039/C5SC01560J

Holzer, A. K., K. Hiebler, F. G. Mutti, R. C. Simon, L. Lauterbach, O. Lenz & W. Kroutil
Asymmetric biocatalytic amination of ketones at the expense of NH3 and molecular hydrogen.
Org Lett.
17:2431-2433 (2015).
doi: 10.1021/acs.orglett.5b01154  [PubMed]

Lenz, O., L. Lauterbach, S. Frielingsdorf & B. Friedrich.
Oxygen-tolerant hydrogenases and their biotechnological potential.
In Biohydrogen (Rögner, M., ed), chapter 4, pp.61-96, De Gruyter, Berlin (2015).

Horch, M., L. Lauterbach, M. A. Mroginski, P. Hildebrandt, O. Lenz & I. Zebger
Reversible active site sulfoxygenation can explain the oxygen tolerance of a NAD+-reducing [NiFe] hydrogenase and its unusual infrared spectroscopic properties.
J. Am. Chem. Soc.
137:2555-2564 (2015).
doi: 10.1021/ja511154y [PubMed]

Karstens, K., S. Wahlefeld, M. Horch, M. Grunzel, L. Lauterbach, F. Lendzian, I. Zebger & O. Lenz
Impact of the iron-sulfur cluster proximal to the active site on the catalytic function of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase.
Biochemistry 54:389-403 (2015).
doi: 10.1021/bi501347u [PubMed]

Lauterbach, L., H. Wang, M. Horch, L. B. Gee, Y. Yoda, Y. Tanaka, I. Zebger, O. Lenz & S. P. Cramer
Nuclear resonance vibrational spectroscopy reveals the FeS cluster composition and active site vibrational properties of an O2-tolerant NAD+-reducing [NiFe] hydrogenase.
Chem. Sci
. 6:1055-1060 (2015).
doi: 10.1039/C4SC02982H

Sigfridsson, K. G., N. Leidel, O. Sanganas, P. Chernev, O. Lenz, K. S. Yoon, H. Nishihara, A. Parkin, F. A. Armstrong, S. Dementin, M. Rousset, A. L. De Lacey & M. Haumann
Structural differences of oxidized iron-sulfur and nickel-iron cofactors in O2-tolerant and O2-sensitive hydrogenases studied by X-ray absorption spectroscopy.
Biochim. Biophys. Acta. 1847:162-170 (2015).
doi: 10.1016/j.bbabio.2014.06.011 [PubMed]



Horch, M., J. Schoknecht, M. A. Mroginski, O. Lenz, P. Hildebrandt & I. Zebger
Resonance Raman spectroscopy on [NiFe] hydrogenase provides structural insights into catalytic intermediates and reactions.
J. Am. Chem. Soc.
136:9870-9873 (2014).
doi: 10.1021/ja505119q [PubMed]

Radu, V., S. Frielingsdorf, S. D. Evans, O. Lenz & L. J. Jeuken
Enhanced oxygen-tolerance of the full heterotrimeric membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha.
J. Am. Chem. Soc. 136:8512-8515 (2014).
doi: 10.1021/ja503138p [PubMed]

Frielingsdorf, S., J. Fritsch, A. Schmidt, M. Hammer, J. Löwenstein, E. Siebert, V. Pelmenschikov, T. Jaenicke, J. Kalms, Y. Rippers, F. Lendzian, I. Zebger, C. Teutloff, M. Kaupp, R. Bittl, P. Hildebrandt, B. Friedrich, O. Lenz & P. Scheerer
Reversible [4Fe-3S] cluster morphing in an O₂-tolerant [NiFe] hydrogenase.
Nat. Chem Biol. 10:378-85 (2014).
doi: 10.1038/nchembio.1500 [PubMed]

Fritsch, J., E. Siebert, J. Priebe, I. Zebger, F. Lendzian, C. Teutloff, B. Friedrich & O. Lenz
Rubredoxin-related maturation factor guarantees metal cofactor integrity during aerobic biosynthesis of membrane-bound [NiFe] hydrogenase.
J. Biol. Chem. 289:7982-7993 (2014).
doi: 10.1074/jbc.M113.544668 [PubMed]



Lauterbach, L. & O. Lenz
Catalytic water production by oxygen-tolerant [NiFe]-hydrogenase during H₂ cycling in the presence of O₂.
J. Am. Chem. Soc. 135:17897-17905 (2013).
doi: 10.1021/ja408420d [PubMed]

Herr, N., J. Ratzka, L. Lauterbach, O. Lenz & M. B. Ansorge-Schumacher
Stability enhancement of an O₂-tolerant NAD+-reducing [NiFe]-hydrogenase by a combination of immobilisation and chemical modification.
J. Mol. Catal. B: Enzym 97: 169–174 (2013).
doi: 10.1016/j.molcatb.2013.06.009

Schäfer, C., B. Friedrich & O. Lenz
Characteristics of a novel, oxygen-insensitive group 5 [NiFe]-hydrogenase in Ralstonia eutropha.
Appl. Environ. Microbiol. 79(17):5137-5145 (2013).
doi: 10.1128/AEM.01576-13 [PubMed]
Highlighted as article of significant interest: doi: 10.1128/AEM.02268-13

Healy, A. J., P. A. Ash, O. Lenz & K. A. Vincent
Attenuated total reflectance infrared spectroelectrochemistry at a carbon particle electrode; unmediated redox control of a [NiFe]-hydrogenase solution.

Phys. Chem. Chem. Phys. 5:7055-7059 (2013).
doi: 10.1039/c3cp00119a [PubMed]

Siebert, E. , M. Horch, Y. Rippers, J. Fritsch, S. Frielingsdorf, O. Lenz, F. Velazquez Escobar, F. Siebert, L. Paasche, U. Kuhlmann, F. Lendzian, M.-A. Mroginski, I. Zebger & P. Hildebrandt
Resonance Raman spectroscopy as a novel tool to monitor the active site of hydrogenases: new insights into structure and reactivity.
Angew. Chem. Int. Ed. Engl. 52:5162-5165 (2013).
doi: 10.1002/anie.201209732 [PubMed]

Lauterbach, L., O. Lenz & K. A. Vincent
H₂-driven cofactor regeneration using NAD(P)+-reducing hydrogenases.

FEBS J. 280:3058–3068 (2013).
doi: 10.1111/febs.12245 [PubMed]

Fritsch, J., O. Lenz & B. Friedrich.
Structure, function and biosynthesis of O₂-tolerant hydrogenases.
Nat. Rev. Microbiol.
11:106-114 (2013).
doi: 10.1038/nrmicro2940 [PubMed]



Bürstel, I., E. Siebert, G. Winter, P. Hummel, I. Zebger, B. Friedrich & O. Lenz.
A universal scaffold for synthesis of the Fe(CN)₂(CO) moiety of [NiFe] hydrogenase.
J. Biol. Chem.
287:38845-38853 (2012).
doi: 10.1074/jbc.M112.376947 [PubMed]

Horch, M., L. Lauterbach, O. Lenz, P. Hildebrandt & I. Zebger.
NAD(H)-coupled hydrogen cycling – structure-function relationships of bidirectional [NiFe] hydrogenases.
FEBS Lett. 586:545-556 (2012).
doi: 10.1016/j.febslet.2011.10.010 [PubMed]

Reeve, H. A., L. Lauterbach, P. A. Ash, O. Lenz & K. A. Vincent.
A modular system for regeneration of NAD cofactors using graphite particles modified with hydrogenase and diaphorase moieties.
Chem. Commun. 48:1589–1591 (2012).
doi: 10.1039/c1cc14826e [PubMed]

Ratzka, J., L. Lauterbach, O. Lenz & M. B. Ansorge-Schumacher.
Stabilisation of the NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 through modification with methoxy-poly(ethylene) glycol.
J. Mol. Catal. B: Enzym 74:219– 223 (2012).
doi: 10.1016/j.molcatb.2011.10.008



Bürstel, I., P. Hummel, E. Siebert, N. Wisitruangsakul, I. Zebger, B. Friedrich & O. Lenz.
Probing the origin of the metabolic precursor of the CO ligand in the catalytic center of [NiFe]-hydrogenase.
J. Biol. Chem. 286:44937-44944 (2011).
doi: 10.1074/jbc.M111.309351 [PubMed]

Frielingsdorf, S., T. Schubert, A. Pohlmann, O. Lenz & B. Friedrich.
A trimeric supercomplex of the oxygen-tolerant membrane-bound [NiFe]-hydrogenase from Ralstonia eutropha H16.
Biochemistry 50:10836-10843 (2011).
doi: 10.1021/bi201594m [PubMed]

Fritsch, J., P. Scheerer, S. Frielingsdorf, S. Kroschinsky, B. Friedrich, O. Lenz & C. M. Spahn.
The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre.
Nature 479:249–252 (2011).
doi: 10.1038/nature10505 [PubMed]
Recommended by the Faculty of 1000

Sezer, M., S. Frielingsdorf, D. Millo, N. Heidary, T. Utesch, M. A. Mroginski, B. Friedrich, P. Hildebrandt, I. Zebger & I. M. Weidinger.
Role of the HoxZ subunit in the electron transfer pathway of the membrane-bound [NiFe]-hydrogenase from Ralstonia eutropha immobilized on electrodes.
J. Phys. Chem. B 115:10368-10374 (2011)
doi: 10.1021/jp204665 [PubMed]

Ratzka, J., L. Lauterbach, O. Lenz & M. B. Ansorge-Schumacher.
Systematic evaluation of the dihydrogen-oxidising and NAD+-reducing soluble [NiFe]-hydrogenase from Ralstonia eutropha H16 as a cofactor regeneration catalyst.
Biocatal. Biotransformation 29:246-252 (2011).
doi: 10.3109/10242422.2011.615393

Lauterbach, L., Z. Idris, K. A. Vincent, O. Lenz.
Catalytic properties of the isolated diaphorase fragment of the NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha.
PLoS ONE 6:e25939 (2011).
doi: 10.1371/journal.pone.0025939 [PubMed]

Lukey, M. J., M. M. Roessler, A. Parkin, R. M. Evans, R. A. Davies, O. Lenz, B. Friedrich, F. Sargent & F. A. Armstrong.
Oxygen-tolerant [NiFe]-hydrogenases: the individual and collective importance of supernumerary cysteines at the proximal Fe S cluster.
J. Am. Chem. Soc. 133:16881-16892 (2011).
doi: 10.1021/ja205393w [PubMed]

Carrieri, D., G. Ananyev, O. Lenz, D. A. Bryant, & G. C. Dismukes.
A sodium ion gradient contributes to energy conservation during fermentation in the cyanobacterium Arthrospira (Spirulina) maxima CS-328.
Appl. Environ. Microbiol. 77:7185-7194 (2011).
doi: 10.1128/AEM.00612-11  [PubMed]

Fritsch, J, S. Löscher, O. Sanganas, E. Siebert, I. Zebger, M. Stein, M. Ludwig, A. L. De Lacey, H. Dau, B. Friedrich, O. Lenz, & M. Haumann.
[NiFe]- and [FeS]-cofactors in the membrane-bound hydrogenase of Ralstonia eutropha investigated by X-ray absorption spectroscopy: insights into O2-tolerant H2 cleavage.
Biochemistry 50:5858-5869 (2011).
doi: 10.1021/bi200367u [PubMed

Fritsch, J., O. Lenz & B. Friedrich.
The maturation factors HoxR and HoxT contribute to oxygen tolerance of membrane-bound [NiFe]-hydrogenase in Ralstonia eutropha H16.
J. Bacteriol. 193:2487-2497 (2011).
doi: 10.1128/JB.01427-10 [PubMed]

Goris, T., A. F. Wait, M. Saggu, J. Fritsch, N. Heidary, M. Stein, I. Zebger, F. Lendzian, F. A. Armstrong, B. Friedrich & O. Lenz.
A unique iron-sulfur cluster is crucial for oxygen tolerance of a [NiFe]-hydrogenase.
Nat. Chem Biol. 7:310-318 (2011).
doi: 10.1038/nchembio.555 [PubMed]
Recommended by the Faculty of 1000

Friedrich, B., J. Fritsch & O. Lenz.
Oxygen-tolerant hydrogenases in hydrogen-based technologies.
Curr. Opin. Biotechnol. 22:358-364 (2011).
doi: 10.1016/j.copbio.2011.01.006 [PubMed]

Lauterbach, L., J. Liu, M. Horch, P. Hummel, A. Schwarze, M. Haumann, K. A. Vincent, O. Lenz & I. Zebger.
The hydrogenase subcomplex of the NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha – Insights into catalysis and redox interconversions.
Eur. J. Inorg. Chem. 2011:1067-1079 (2011).



Horch, M., L. Lauterbach, M. Saggu, P. Hildebrandt, F. Lendzian, R. Bittl, O. Lenz & I. Zebger.
Probing the active site of an O2-tolerant NAD+-reducing [NiFe]-hydrogenase from Ralstonia eutropha H16 by in situ EPR and FTIR spectroscopy.
Angew. Chem. Int. Ed. Engl. 49:8026-8029 (2010).
doi: 10.1002/anie.201002197 [PubMed]

Carrieri, D., D. Momot, I. A. Brasg, G. Ananyev, O. Lenz, D. A. Bryant, G. C. Dismukes.
Boosting autofermentation rates and product yields with sodium stress cycling: application to production of renewable fuels by cyanobacteria.
Appl. Environ. Microbiol. 76:6455-6462 (2010).
doi: 10.1128/AEM.00975-10 [PubMed]

Saggu, M., M. Ludwig, B. Friedrich, P. Hildebrandt, R. Bittl, F. Lendzian, O. Lenz & I. Zebger.
Impact of amino acid substitutions near the catalytic site on the spectral properties of an O2-tolerant membrane-bound [NiFe] hydrogenase.
ChemPhysChem. 11:1215-1224 (2010).
doi: 10.1002/cphc.200900988 [PubMed]

Löscher, S., A. Gebler, M. Stein, O. Sanganas, T. Buhrke, I. Zebger, H. Dau, B. Friedrich, O. Lenz & M. Haumann.
Protein-protein complex formation affects the Ni-Fe and Fe-S centers in the H2-sensing regulatory hydrogenase from Ralstonia eutropha H16.
ChemPhysChem. 11:1297-1306 (2010).
doi: 10.1002/cphc.200901007 [PubMed]

Lenz, O., M. Ludwig, T. Schubert, I. Bürstel, S. Ganskow, T. Goris, A. Schwarze & B. Friedrich.
H2 conversion in the presence of O2 as performed by the membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha.
ChemPhysChem. 11:1107-1119 (2010).
doi: 10.1002/cphc.200901002 [PubMed]

Saggu, M., C. Teutloff , M. Ludwig, M. Brecht, M. E. Pandelia, O. Lenz, B. Friedrich, W. Lubitz, P. Hildebrandt, F. Lendzian & R. Bittl.
Comparison of the membrane-bound [NiFe] hydrogenases from R. eutropha H16 and D. vulgaris Miyazaki F in the oxidized ready state by pulsed EPR.
Phys. Chem. Chem. Phys. 12:2139-2148 (2010).
doi: 10.1039/B922236G

Schwarze, A., M. J. Kopczak, M. Rögner & O. Lenz.
Requirements for construction of a functional hybrid complex of photosystem I and [NiFe]-hydrogenase.
Appl. Environ. Microbiol. 76:2641-2651 (2010).
doi: 10.1128/AEM.02700-09 [PubMed]



Krassen, H., A. Schwarze, B. Friedrich, K. Ataka, O. Lenz & Joachim Heberle.
Photosynthetic hydrogen production by a hybrid complex of photosystem I and [NiFe]-hydrogenase.
ACS Nano. 3:4055-4061 (2009).
doi: 10.1021/nn900748j [PubMed]

Schwartz, E., B. Voigt, D. Zühlke, A. Pohlmann, O. Lenz, D. Albrecht, A. Schwarze, Y. Kohlmann, C. Krause, M. Hecker & B. Friedrich.
A proteomic view of the facultatively chemolithoautotrophic lifestyle of Ralstonia eutropha H16.
Proteomics. 9:5132-5142 (2009).
doi: 10.1002/pmic.200900333  [PubMed]

Cracknell, J.A., A. F. Wait, O. Lenz,B. Friedrich & F. A. Armstrong.
A kinetic and thermodynamic understanding of O2 tolerance in [NiFe]-hydrogenases.
Proc. Natl. Acad. Sci. U. S. A. 106:20681-20686 (2009).
doi: 10.1073/pnas.0905959106 [PubMed]

Krauße, D., K. Hunold, B. Kusian, O. Lenz, J. Stülke, B. Bowien & J. Deutscher.
Essential Role of the hprK gene in Ralstonia eutropha H16.
J. Mol. Microbiol. Biotechnol. 17:146-152 (2009).
doi: 10.1159/000233505 [PubMed

Saggu, M., I. Zebger, M. Ludwig, O. Lenz, B. Friedrich, P. Hildebrandt & F. Lendzian.
Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe]-hydrogenase of Ralstonia eutropha H16.
J. Biol. Chem. 284:16264–16276 (2009).
doi: 10.1074/jbc.M805690200 [PubMed]
Recommended by the Faculty of 1000

Ludwig, M., T. Schubert, I. Zebger, N. Wisitruangsakul, M. Saggu, A. Strack, O. Lenz, P. Hildebrandt & B. Friedrich.
Concerted action of two novel auxiliary proteins in assembly of the active site in a membrane-bound [NiFe]–hydrogenase.

J. Biol. Chem. 284:2159-2168 (2009).
doi: 10.1074/jbc.M808488200 [PubMed]

Ludwig, M., J. A. Cracknell, K. A. Vincent, F. A. Armstrong & O. Lenz.
Oxygen-tolerant H2 oxidation by membrane-bound [NiFe]–hydrogenase of Ralstonia species - Coping with low-level H2 in air.
J. Biol. Chem. 284:465-477 (2009).
doi: 10.1074/jbc.M803676200 [PubMed]

Wisitruangsakul, N., O. Lenz, M. Ludwig, B. Friedrich, F. Lendzian, P. Hildebrandt & I. Zebger.
Monitoring catalysis of the membrane-bound hydrogenase from Ralstonia eutropha H16 by surface-enhanced infrared absorption spectroscopy.
Angew. Chem. Int. Ed. Engl. 48:611-613 (2009).
doi: 10.1002/anie.200802633 [PubMed]



Ludwig, M., A. Schwarze & O. Lenz.
Knallgas unter Kontrolle: O2-tolerante Hydrogenasen und ihre Anwendung.
Biospektrum 5-2008:477-479 (2008).

Goldet, G., A. F. Wait, J. A. Cracknell, K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich & F. A. Armstrong.
Hydrogen production under aerobic conditions by membrane-bound hydrogenases from Ralstonia species.
J. Am. Chem. Soc. 130:11106-13 (2008).
doi: 10.1021/ja8027668 [PubMed]

Cracknell, J. A., K. A. Vincent, M. Ludwig, O. Lenz, B. Friedrich & F. A. Armstrong.
Enzymatic oxidation of H2 in atmospheric O2: the electrochemistry of energy generation from trace H2 by aerobic microorganisms.
J. Am. Chem. Soc. 130:424-425 (2008).
doi: 10.1021/ja078299+ [PubMed]

Schubert, T., O. Lenz, E. Krause, R. Volkmer & B. Friedrich.
Chaperones specific for the membrane-bound [NiFe]-hydrogenase interact with the Tat signal peptide of the small subunit precursor in Ralstonia eutropha H16.
Mol. Microbiol. 66:453-467 (2008).
doi: 10.1111/j.1365-2958.2007.05933.x [PubMed



Lenz, O., I. Zebger, J. Hamann, P. Hildebrandt & B. Friedrich.
Carbamoylphosphate serves as the source of CN-, but not of the intrinsic CO in the active site of the regulatory [NiFe]-hydrogenase from Ralstonia eutropha.
FEBS Lett. 581:3322-3326 (2007).
doi: 10.1016/j.febslet.2007.06.027 [PubMed]

Gebler, A., T. Burgdorf, A. L. De Lacey, O. Rüdiger, A. Martinez-Arias , O. Lenz & B. Friedrich.
Impact of alterations near the [NiFe] active site on the function of the H2 sensor from Ralstonia eutropha.
FEBS J. 274:74-85 (2007).
doi: 10.1111/j.1742-4658.2006.05565.x [PubMed]



Vincent, K. A., J. A. Cracknell, J. R. Clark, M. Ludwig, O. Lenz, B. Friedrich & F. A. Armstrong.
Electricity from low-level H2 in still air-an ultimate test for an oxygen tolerant hydrogenase.
Chem. Commun. (Camb). 28:5033-5035 (2006).
doi: 10.1039/B614272A [PubMed]

Ihara, M., H. Nishihara, K.-S. Yoon, O. Lenz, B. Friedrich, H. Nakamoto, K. Kojima, D. Honma, T. Kamachi & I. Okura.
Light-driven hydrogen production by a hybrid complex of a [NiFe]-hydrogenase and the cyanobacterial photosystem I.
Photochem. Photobiol. 82:676-682 (2006).
doi: 10.1562/2006-01-16-RA-778 [PubMed]



Vincent, K. A., A. Parkin, O. Lenz, S. P. J. Albracht, J. C. Fontecilla-Camps, R. Cammack, B. Friedrich & F. A. Armstrong.
Electrochemical definitions of O2 sensitivity and oxidative inactivation in hydrogenases.
J. Am. Chem. Soc. 127:18179-18189 (2005).
doi: 10.1021/ja055160v [PubMed]

Winter, G., T. Buhrke, O. Lenz, A. K. Jones & B. Friedrich.
A model system for [NiFe]-hydrogenase maturation studies: purification of an active site containing hydrogenase large subunit without small subunit.
FEBS Lett. 579:4292-4296 (2005).
doi: 10.1016/j.febslet.2005.06.064 [PubMed]

Vincent, K. A., J. A. Cracknell, O. Lenz, I. Zebger, B. Friedrich & F. A. Armstrong.
Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels.
Proc. Natl. Acad. Sci. U. S. A. 102:16951-16954 (2005).
doi: 10.1073/pnas.0504499102
Recommended by the Faculty of 1000

Lenz, O., A. Gleiche, A. Strack & B. Friedrich.
Requirements for heterologous production of a complex metalloenzyme: the membrane-bound [NiFe] hydrogenase.
J. Bacteriol. 187:6590-6595 (2005).
doi: 10.1128/JB.187.18.6590-6595.2005 [PubMed]

Burgdorf, T., O. Lenz, T. Buhrke, E. van der Linden, A. K. Jones, S. P. J. Albracht & B. Friedrich.
The [NiFe]-hydrogenases of Ralstonia eutropha H16: modular enzymes for oxygen tolerant biological hydrogen oxidation.
J. Mol. Microbiol. Biotechnol. 10:181-196 (2005).
doi: 10.1159/000091564 [PubMed]

Friedrich, B., T. Buhrke, T. Burgdorf & O. Lenz.
A hydrogen-sensing multiprotein complex controls aerobic hydrogen metabolism in Ralstonia eutropha.
Biochem. Soc. Trans. 33:97-101 (2005).
[Journal] [PubMed]

Buhrke, T., O. Lenz, N. Krauß & B. Friedrich.
Oxygen tolerance of the H2-sensing regulatory [NiFe] hydrogenase from Ralstonia eutropha is based on limited access of oxygen to the active site.
J. Biol. Chem. 280:23791-23796 (2005).
doi: 10.1074/jbc.M503260200 [PubMed]

Buhrke, T., S. Löscher, O. Lenz, E. Schlodder, I. Zebger, L. K. Andersen, P. Hildebrandt, W. Meyer-Klaucke, H. Dau, B. Friedrich & M. Haumann.
Reduction of unusual iron-sulfur clusters in the H2-sensing regulatory Ni-Fe hydrogenase from Ralstonia eutropha H16.
J. Biol. Chem. 280:19488-19495 (2005).
doi: 10.1074/jbc.M500601200 [PubMed]



Jones, A. K., O. Lenz, A. Strack, T. Buhrke & B. Friedrich.
NiFe hydrogenase active site biosynthesis: identification of Hyp protein complexes in Ralstonia eutropha.
Biochemistry. 43:13467-13477 (2004).
doi: 10.1021/bi048837k [PubMed]

Buhrke, T., O. Lenz, A. Porthun & B. Friedrich.
The H2-sensing complex of Ralstonia eutropha: interaction between a regulatory [NiFe] hydrogenase and a histidine protein kinase.
Mol. Microbiol. 51:1677-1689 (2004).
doi: 10.1111/j.1365-2958.2003.03933.x [PubMed]



Lenz, O., M. Bernhard, T. Buhrke, E. Schwartz & B. Friedrich.
The hydrogen-sensing apparatus in Ralstonia eutropha.
J. Mol. Microbiol. Biotechnol. 4:255-262 (2002).



Lenz, O. & B. Friedrich.
Bakterielle Wasserstoff-Sensoren.
Biospektrum 6:515-520 (2001).

Friedrich, B., P. M. Vignais, O. Lenz & A. Colbeau.
Regulation of hydrogenase gene expression.
In Hydrogen as a Fuel (Cammack, R., Frey, M. & Robson, R., eds), pp. 33-56, Taylor & Francis, London, New York (2001).



Kleihues, L., O. Lenz, M. Bernhard, T. Buhrke & B. Friedrich.
The H2 sensor of Ralstonia eutropha is a member of the subclass of regulatory [NiFe] hydrogenases.
J. Bacteriol. 182:2716-2724 (2000).

Pierik, A. J., M. Schmelz, O. Lenz, B. Friedrich & S. P. J. Albracht.
Characterization of the active site of a hydrogen sensor from Alcaligenes eutrophus.
FEBS Lett. 438:231-235 (1998).

Lenz, O. & B. Friedrich.
A novel multicomponent regulatory system mediates H2 sensing in Alcaligenes eutrophus.
Proc. Natl. Acad. Sci. U. S. A. 95:12474-12479 (1998).

Lenz, O., A. Strack, A. Tran-Betcke & B. Friedrich.
A hydrogen-sensing system in transcriptional regulation of hydrogenase gene expression in Alcaligenes species.
J. Bacteriol. 179:1655-1663 (1997).

Lenz, O., E. Schwartz, J. Dernedde, M. Eitinger & B. Friedrich.
The Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
J. Bacteriol. 176:4385-4393 (1994).

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