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Open call for PhD candidate

Lupe

We are searching for a PhD candidate at our group funded by EC2: Interested in H2-powered biocatalysis and skilled in molecular biology and bioinformatics? Apply for a scholarship/position (proposal no.11) at: https://ec2-big-nse.de/applications Joint project with Maria Andrea Mroginski, Kylie Vincent, Sandy Schmidt and many others. 

New Bachelor student

Lupe

Great, when students return to us! Michelle K. will work together with Ari Satanowski and Steffen Lindner from Bar-Even lab to design autotropic E. coli.

Biological methane conversion

Lupe

Pushing biotechnological approaches with soluble methane monooxygenase: We welcome Marta S. and Domenic Z. in our team! Thanks to TU Berlin for internal funding.

New team member

Lupe

We welcome Valentin Petrich to our team for studies about electro-driven synthesis of fine chemicals. Thanks to BMBF for funding in the framework of “Neue Produkte für die Bioökonomie and pushing our research to application.

Great PhD defence by Ammar

Huge congratulations to Ammar for defending his PhD. Through great commitment and great ideas, Ammar has pushed the field of cofactor regeneration and biocatalyis with four papers published in top journals. I wish him all the best in his future career!

H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions

Lupe

Check out our newest work on "H2 as a fuel for flavin- and H2O2-dependent biocatalytic reactions" in Chem Commun. Thanks to Frank Hollamm and Caroline Paul for the great collaboration!

ChemViews Highlight

Nice ChemViews highlight associated with our study published in Angewandte Chemie about bioelectrochemical synthesis of N-heterocycles together with B. Nestl and U.-P. Apfel: 

https://www.chemistryviews.org/details/ezine/11242978/Bioelectrochemical_Synthesis_of_N-Heterocycles.html

Marie Curie-training network

Lupe

Thanks to all our partners and MSCA for the success of our ConCO2rde ITN-EJD-training network on i.a. H2-driven biotransformations and CO2 fixation! Looking forward to the new collaborations! 

ec.europa.eu/research/mariecurieactions/news/ITN-2020-call-results_en

 

 

Back to lab work

Lupe

Good news: lab work in my group has started again under Covid19 safety conditions. I am happy that studies on H2-driven biotransformations, biomethane and Light-driven H2 production can continue again!

Front Cover

Lupe

Our study about "Powering artificial enzymatic cascades with electrical energy" has made it as a "hot paper" on the front cover of Angew. Chem. Int. Ed.! Great cooperation with B. Nestl and U.-P. Apfel! https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.202005732

Powering artificial enzymatic cascades with electrical energy

Ammar Al-Shameri, Marie-Christine Petrich, Kai junge Puring, Ulf-Peter Apfel, Bettina M. Nestl and Lars Lauterbach

We developed a scalable platform that employs electrolysis for an in vitro synthetic enzymatic cascade in a continuous flow reactor. Both H2 and O2 were produced by electrolysis and transferred via a gas permeable membrane into the flow system. The membrane enabled the separation of the electrolyte from the biocatalysts in the flow system, where H2 and O2 served as electron mediators for the biocatalysts. We demonstrated the production of methylated N‑heterocycles from diamines with up to 99% conversion yield as well as excellent regioselective labeling with stable isotopes. Our platform can be applied for a broad panel of oxidoreductases to exploit electrical energy for the synthesis of fine chemicals. 

Angew. Chem. Int. Ed. 2020| Accepted Manuscript | doi.org/10.1002/anie.202001302

Lupe

New students

Dienstag, 11. Februar 2020

We welcome Nadine Brandt and Michelle Krause to our team for protein-protein interactions studies of engineered hydrogenases. 

Forum Junge Spitzenforscher, Klimawandel

Lupe

Unsere Idee zur "Elektrogetriebene Produktion von Feinchemikalien" ist zum Finalbeitrag des diesjährigen Forum Junge Spitzenforscher mit dem Thema Klimawandeln gekürt wurden.  Ammar Al-Shameri, Bettina Nestl und Lars Lauterbach freuen sich auf die öffentliche Abschlussveranstaltung in Berlin am 20.11.2019.  Zuschauer können sich über http://forumjungespitzenforscher.de/ anmelden. 

We welcome Toan Nguyen to our our team for a project about developing a biological H2 sensor! 

 

 

Cascade Biotransformation to Access 3-Methylpiperidine in Whole Cells

Lupe

Niels Borlinghaus+,[a] Leonie Weinmann+,[a] Florian Krimpzer,[b] Philipp N. Scheller,[a]
Ammar Al-Shameri,[c] Lars Lauterbach,[c] Anne-Sophie Coquel,[b] Claus Lattemann,[b]
Bernhard Hauer,[a] and Bettina M. Nestl*[a]

a] Universität Stuttgart, Institute of Biochemistry and Technical Biochemistry

[b] Sanofi Chimie, Pharmaceutics Development Platform

[c] Technical University of Berlin, Institute of Chemistry

Synthesis of 3-methylpiperidine from 1,5-diamino-2-meth ylpentane in preparative scale is reported by using recombinant Escherichia coli cells expressing a variant of the diamine oxidase from Rhodococcus erythroprolis and an imine reductase from Streptosporangium roseum. Optimization of process parameters for cultivation and bioconversion led to substantial improve ments in the initial laboratory procedure. The transformation of the methyl-substituted diamine substrate to the N-heterocyclic product was successfully scaled-up from shake-flask to a 20 L bioreactor with increased substrate concentrations. Remarkably, we obtained 67% of 3-methylpiperidine product from 140 g substrate within 52 h.

ChemCatChem 2019, 11, 1 –6  

 



Synthesis of N-heterocycles from diamines via H2-driven NADPH recycling in the presence of O2

Lupe

Ammar Al-Shameri, Niels Borlinghaus, Leonie Weinmann, Philipp N. Scheller, Bettina M. Nestl and Lars Lauterbach

Enzymatic cascades enable the regioselective and stereroselective synthesis of fine chemicals and pharmaceutics in a series of coupled reactions, which is of core interest of UniSysCat. In a joined collaboration, the groups of Lauterbach and Nestl designed an enzymatic cascade for the H2-driven synthesis of nitrogen-heterocycles. Saturated nitrogen-heterocycles, especially pyrrolidines and piperidines, are attractive scaffolds for agrochemicals and pharmaceuticals. The newly developed enzymatic cascade included an O2-dependent putrescine oxidase, a NADPH-dependent imine reductase and an O2-tolerant NADP+-reducing hydrogenase. The incorporation of a hydrogenase made the reducing power of H2 available for biosynthesis. Enzyme engineering approaches were combined to optimize the specificity and activity of the putrescine oxidase from Rhodococcus erythropolis for the transformation of substituted diamines to the corresponding pyrrolidines and piperidines. The one-pot reaction resulted in up to 97% product formation. Furthermore, high selectivities up to 93% ee were obtained by exploiting the kinetic resolution of the putrescine oxidase. The prevention of organic solvents, toxic compounds or purification of intermediates demonstrates the “greeness” of this approach.

Green Chem. 2019 | Accepted Manuscript | DOI:10.1039/C8GC03798A

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